Following a centrifugation at 12, 000 g for 30 minutes, the absorbance of EBD in the supernatant was measured at 620 nm the absorbance maximum for EBD and corrected for a contamination of heme pigments OD740 nm as follows OD620 corrected OD620 1 lasix diuretic side effects With a rapidly changing world, and increasingly worrying statistics about child health and well being in the UK, we really can t afford not to